Christian Nathan

Christian Nathan

Profile

I joined the University of Leeds in 2015 as a PhD student. I obtained my Master's of Science in Biology from Carleton University (2014) in Ottawa, Canada and prior to this I obtained my undergraduate degree in Biology and Biochemistry from the University of Winnipeg (2011).

Research interests

My current research focuses on the molecular clock and understanding its influence on the sympathetic nervous system and the cardiovascular system. My main project focuses on determining the link between the molecular clock and sympathetic preganglionic neurons, a spinal cord cell type.

Skills and techniques:

  • Home Office license obtained (2016). In vivo work includes tracing studies, perfusion fixation, and perfusion for slices (mice).
  • Preparation of tissue via vibrating microtome and cryostat, immunohistochemistry, confocal microscopy.
  • Fresh fixation preparation of tissue for RNA extraction, kit-based and phenol-chloroform RNA extraction, reverse transcription, quantitative polymerase chain reaction, design of PCR primers, agarose gel electrophoresis.
  • Kit-based RNA extraction from single cell or low RNA input, bead-based cDNA purification for single cell RNA-seq.

Qualifications

  • BSc Biology and Biochemistry
  • MSc - Biology, Neuroethology

Research groups and institutes

  • Neuroscience