Professor Lars Jeuken
- Position: Professor of Molecular Biophysics
- Areas of expertise: bioinorganic chemistry; bionanotechnology; bioenergetics; membrane biophysics; bioelectrochemistry; biosensing and biosensors
- Email: L.J.C.Jeuken@leeds.ac.uk
- Phone: +44(0)113 343 3829
- Location: Bioincubator 7.19 Garstang
- Website: The Jeuken Group | Twitter | LinkedIn | Researchgate | ORCID
I graduated from the University of Utrecht in the Netherlands (1995) and obtained my PhD in metalloproteins under the supervision of Prof. G.W. Canters at Leiden University (2001). After my PhD, I moved to the United Kingdom to work with Prof. F.A. Armstrong at the University of Oxford (2000-2002) on the electrochemical characterisation of metalloproteins.
I was awarded a BBSRC David Phillips 5-year fellowship which I took up at the School of Physics & Astronomy at the University of Leeds (2002-2007) to work on the modification and characterisation of electrode surfaces for bioelectrochemistry, for which I received the British Biophysical Society (BBS) Young Investigator Award in 2006. In 2007 I moved to the School of Biomedical Sciences at Leeds, where after an ERC fellowship (2012-2016) I became Professor in Molecular Biophysics.
Our aim is to develop novel materials, including nanoparticles, that interact directly with redox enzymes, cell membranes or whole bacteria. This technology either serves as a platform for the development of diagnostics/biosensors and fuel cells or to study the catalytic mechanism of redox-active membrane proteins. The electrode materials, which include ''membrane-modified electrodes" (see Figure), are characterised with a broad spectrum of biophysical tools, including Atomic Force Microscopy (AFM) and electrochemistry. Using electrochemical tools, the electron transport activity of redox enzymes in the membranes is directly converted into a measurable current enabling the detailed study of respiratory enzymes. By combining our electrochemical methods with fluorescent techniques, we aim to obtain information about processes normally not detected by electrochemistry, such as proton pumping or the redox state of single redox site in a multisite enzyme.
Redox-active membrane enzymes
We have modified electrodes with lipid membranes in which the membranes either adopt a planar geometry (see Figure) or liposomes are immobilised intact on the surface. This allows us to study redox-active membrane proteins in their native environment. We use these systems to study the catalytic activity and proton pumping activity of an ubiquinol oxidase from E. coli, cytochrome bo3. Currently, we have three projects in this area.
Single-enzyme studies of a proton pump
By modifying the electrode surfaces, membrane vesicles can be adsorbed on the surface that stay intact. By loading these vesicles with fluorescent probes we can simultaneously control the activity of the ubiquinol oxidase, cytochrome bo3 (using electrochemical methods) and measure its proton pumping activity (see also the ERC project 'MEME'). Currently, this experimental setup is used to study the proton pumping activity of a heme-copper oxidase and complex I at the single-enzyme level.
Respiration in Shewanella oneidensis MR-1
The γ-proteobacterium Shewanella oneidensis MR-1 (MR-1) has become a model organism for the development of microbial electrochemical systems (MESs), such as microbial fuel cells (MFCs). Currently, the electron transfer pathways and performance of MESs are most often optimised by systematically varying fuel supplements and/or electrode. However, to enable an informed or designed enhancement of MESs, biochemical insight is needed into the determinants of electron flux within the extensive network of redox-active proteins inside the microbes that make up MESs. Porin:cytochrome complexes, e.g., MtrCAB (Fig.1) span the outer-membrane of Shewanella oneidensis MR-1 and provide a conduit for electron exchange between the cell’s interior and its exterior (i.e. the electrode). To engineer and optimise the electron flux on the periplasmic side of this porin:cytochrome complex, we aim to create a deeper understanding of the extensive network of redox active proteins inside MR-1.
Membrane-bound hydrogenase (MBH)
MBHs are hydrogen/proton converting enzymes that donate to or extract electrons from the quinol pool in the lipid membrane as part of the active metabolism of some microbes such as Ralstonia eutropha. These enzymes are extensively studied using electrochemical methods, but invariably only the two hydrophilic subunits are used, omitting a third membrane subunit that interacts with the quinone pool. In this project, the whole heterotrimeric MBH is placed in the planar lipid membrane on the electrode surface (see image above) and the activity and other properties (such is deactivation) are characterised using electrochemical methods.
New supported membrane platforms for the study of transporters and membrane metallo-enzymes
For a long time it has been realised that the solid support unerneath supported bilayer will interact with membrane proteins incorported and possibly disrupt the structure or otherwise impair the function the membrane proteins. Several solutions to this problem have been proposed in literature, including a so-called double supported membrane. However, few have been actually tested, let alone used in application with medically relevant membrane proteins. In this project, we are testing the double and multi layer platform in the study of transporters and membrane metallo-enzymes. In one project, we are using the solid-supported membrane platform from the Surfe2r technology that can be used for the biophysical characterisation of ion pumps and transporters. In a second project, we are expanding multi-membrane platforms for the electrochemical study of quinone dehydrogenases
Bionanotechnology: Exploiting interactions between nanoparticles and cell membranes
Exploiting Transmembrane Cytochromes for Solar Energy Conversion
There is vast potential for renewable fuel and electricity generation through the photochemical conversion of solar to chemical energy. We wish to tap into what is a largely unused power supply and develop the basic principles for a novel biotechnology that will harness solar energy using a biological synthetic approach, in which a bacterial respiratory machinery is coupled to inorganic photosensitisers. Our biosynthetic approach is inspired by plant photosynthesis where solar energy is absorbed by the special pair of chlorophylls (the photosensitisers) and, ultimately, converted to chemical energy. Our ambition is to overcome the major drawbacks of most artificial homogenous photosynthetic systems: a short-lived charge separated state, which is due to the failure to spatially decouple the reductive and the oxidative sites required to sustain charge separation. In plant photosynthesis, the latter is achieved by rapid transmembrane electron transfer. In this project, which is a collaboration with the Universities of East Anglia and Cambridge, transmembrane charge separation is achieved by exploiting the membrane-spanning electron-transfer conduits of the microbe Shewanella oneidensis MR-1, where the cytochrome conduits play an integral part of their ability to respire on inorganic minerals. As a proof of principle, we aim to photocatalyse the reduction of protons to hydrogen, using both inorganic (platinum) and biological (hydrogenase) catalysts. The resulting photocatalyst adopts the principles of natural photosynthesis (light harvesting, charge separation and catalysis) in a device that spatially separates the sites of photoexcitation and oxidation to that from reduction (hydrogen evolution). The image illustrates a cytochrome condiut protein (MtrF) coupled to a inorganic photosensitiser (TiO2). The assembly is adorbed on a gold electrode and electrochemistry is used to characterise the electron transfer rates between (photoexcited) TiO2 and MtrF.
Microbial fuel cells
Recently, an intense interest has emerged in microorganisms that generate electrical current from organic matter in microbial fuel cells (MFCs). MFCs have the ability to generate a clean and renewable energy and to generate continuous power in specialised environments like the bottom of the sea bed or the human body. However, one of the greatest bottlenecks in MFC development is their limited power output, which is still insufficient to drive most electronic devices in society. The limitation in power output is mostly due to the slow rate of electron transfer from the microbes to the anode of the MFC. Using our technology, we aim to 'electrically' connect bacteria in biofilms to the electrode (anode) via nanowires and thereby enhancing the electron transfer rate between microbe and the electrode, and thus the power output of the MFC. To analyse the interactions between bacteria such as Shewanella oneidensis MR-1 and chemically-modified nanoparticles, we make use of quantum dots, which are semi-conducting nanoparticulates that are strongly fluorescent and thus easy to image (see Figure).
Novel diagnostics to fight antimicrobial resistance (AMR)
The lack of instant and accurate diagnostic tools for infectious diseases leads to unnecessary antimicrobial prescribing. In primary and secondary care settings, this contributes directly and significantly to antimicrobial resistance (AMR). Additionally, it can select for potentially life-threatening infections, such as Clostridium difficile infection (CDI) and methicillin resistant Staphylococcus aureus (MRSA) infection, thus contributing indirectly but still significantly to AMR. To address this challenge, our group is contributing to a programme that will develop innovative approaches for the next generation of biosensors which provide rapid and highly reliable tools for infection diagnosis. Current biosensor development uses passive capture molecules such as antibodies to bind biomarkers, despite the most successful biosensor (glucose sensor) relying on active enzymes for sensitivity and reliability. In our project, we generate new classes of active capture molecules that will enable highly sensitive, reliable and instant diagnostics for both primary and secondary care settings. Our vision is to integrate these biosensors with sample handling fluidics to deliver a pipeline of point-of-care diagnostics, which will distinguish between viral and bacterial infections, allow confirmation of specific bacterial pathogens and detect antimicrobial resistance mechanisms.<h4>Research projects</h4> <p>Any research projects I'm currently working on will be listed below. Our list of all <a href="https://biologicalsciences.leeds.ac.uk/dir/research-projects">research projects</a> allows you to view and search the full list of projects in the faculty.</p>
- Fellow of the Royal Society of Chemistry (FRSC)
- Biochemical Society
- International Society of Electrochemistry (ISE)
- British Biophysical Society (BBS)
- American Chemical Society (ACS)
Postgraduate studentship areas:
We have various funded PhD positions available throughout the year. See our Research projects for more information. If you are interested in doing a PhD project, please contact Lars Jeuken (L.J.C.Jeuken@leeds.ac.uk).
Undergraduate final-year project topics (BSc and MBiol):
- Infection diagnostics and biosensors (lab project)
- Bioenergetics of complex I (lab project)
- Data Management in Undergraduate Research Projects (educational project)
- Mitochondrial bioenergetics (critical review)
- Oxidative stress and Neurodegenerative disease (critical review)
Other undergraduate teaching:
- I teach on several modules for programmes from the School of Biomedical Sciences (BSc Medical Science) and the School of Cellular and Molecular Biology (BSc Biology Sciences, BSc Biochemistry)
- My topics range from Membrane Transport to Bioinorganic Chemistry
Research groups and institutes
- Integrative Membrane Biology