Bio-imaging equipment

Stimulated emission depletion (STED) microscopy is a super-resolution microscopy technique. It uses an intense laser light in the outer edges of the diffraction limited excitation focus to selectively deactivate fluorophores, returning them to the ground state. This minimises the area of excitation at the focal point, increasing resolution. The STEDYcon system converts an epifluorescent microscope into a multicolour STED system.

Capabilities

• Epi-fluorescent imaging of fixed and live cells
• STED imaging of fixed and live cells
• 2 colour STED imaging
• 4 colour epi-fluorescent / confocal imaging
• Resolution ~40nm
• Z-Piezo for fast accurate Z-stacks

Microscope

• Zeiss Axio-Observer Z1
• Photometrics Prime BSI CMOS camera.
• Temperature controlled chamber for live cell imaging
• STEDYcon system for STED imaging

• 100x/1.4 objective for STED imaging

Lasers

• 488nm
• 561nm
• 640nm
• 775nm (STED laser)

Detection

• 650-700nm
• 578-627nm
• 505-545nm

Location: L.C.Miall 5.08, Faculty of Biological Sciences.