1. Faculty of Biological Sciences
  2. Facilities
  3. Bio-imaging equipment

Bio-imaging equipment

Zeiss AxioScan Z.1 Slide Scanner

The automated slide scanner enables high-quality and high through-put imaging of whole slides. It can be used for fixed cells, tissue sections or tissue microarrays and is capable of both bright-field and fluorescence imaging.   

Capabilities

  • Automated system that allows high through-put of large numbers of slides
  • Capable of imaging up to 100 slides in a single run
  • Imaging of histological / immunohistochemically stained sections
  • Imaging of Fluorescently labelled samples
  • Tiling of whole tissue sections
  • Z-stack (3D) imaging of whole tissue sections

Microscope

  • Equipped with Colibri7 LED light source giving illumination at 385nm, 430nm, 457nm, 511nm, 555nm, 590nm, 630nm
  • 2 cameras: Hitachi HV-F202 and Hamamatsu Orca Flash 4.0v2

Objectives

  • Fluar 5x/0.25
  • Plan-Apoochromat 10x/0.45
  • Plan-Apochromat 20x/0.8
  • Plan-Apochromat 40x/0.95

Filter Sets

  • [Filter set 49] DAPI - Ex365 Em445/50
  • [Filter set 38] GFP - Ex470/40 EmBP525/50
  • [Filter set 43] DeRed - Ex545/25 Em605/70
  • [Filter set 64] mPlum - Ex 587/25 Em647/70
  • [Filter set 50] Cy5 - Ex640/30 Em690/50
  • [Filter set 92] DAPI, GFP, mCherry  – Ex385/30, 469/38, 591/27. TBP Em25/30+524/50+688/145
  • [Filter set 90] Quad: DAPI, GFP, CY3 ,Cy5 – Ex385/30, 469/38, 555/30, 631/33. QBP Em425/30+514/30+592/25+709/100

 

Location: Faculty of Biological Sciences, L.C.Miall 5.11a