Abberior SAFe360 SMLM Microscope

The single molecule light microscopy (SMLM) is a super-resolution microscope technique. Enabling super-resolution techniques such as PALM and STORM as well as single molecule techniques such as TIRF microscopy. Resolution can be achieved down to 20nm.

Capabilities

• Epifluorescence (Epi)
• HiLo
• TIRF
• Spectral de-mixing

Saved Configurations

• SAFe360 – Simulataneous 2 colours – Nanoscopy – 2D
• SAFe360 – Single Colour – Nanoscopy – 2D
• SAFe360 – Single Colour – Nanoscopy – 3D – Astigmatism
• SAFe360 – Spectral Demixing – Nanoscopy – 2D

Imaging Modes

• 2D SMLM – single colour / two colour
• 3D SMLM – single colour / two colour

Microscope

• Olympus IX83
• Manual Stage
• 2x Orca Fusion BT Cameras
• ZDC
• Neo Software

Lasers

• 405nm
• 488nm
• 532nm
• 561nm
• 640nm

Detection Filters

• ET700/75
• ET600/50m
• QUAD 446/510/581/703-25
• ET525/50m
• ET445/58
• QUAD 405/488/561/640

Location: Bioimaging Facility, LC Miall 5.10, Faculty of Biological Sciences.