Bio-imaging equipment

A widefield microscope able to produce high quality images of both fluorescently labelled fixed and live cell samples. Deconvolution improves the resolution of images by computationally removing out of focus light through a mathematical algorithm.

Capabilities

• Epi-fluorescent imaging of fixed and live cells
• Differential Interference Contrast (DIC) imaging
• High degree of sensitivity
• Ideal for imaging light sensitive samples
• Temperature control for long time lapse imaging of live cells
• Precision XYZ stage for multi-point imaging and tiling
• Improved image resolution via optimised deconvolution processing

Microscope

• Olympus IX83
• Motorised XYZ stage for tile scanning, multi-point imaging and slide scanning. 
• Photometrics Prime BSI CMOS camera.
• Temperature controlled chamber for live cell imaging.
• CellSense image acquisition and analysis software

Objectives

• 10x/0.40, U Plan Apo
• 20x/0.75, U PlanS Apo
• 40x/1.35 Oil Iris, U Apo/340 (oil)
• 60x/1.4 Oil, Plan Apo (oil)
• 100x/1.35 Oil Iris, U Plan Apo (oil)

Filter Sets

• DAPI (ex 365/10) em 440/40
• GFP (ex 482/24) em 530/40
• RFP (ex 545/10) em 600/50
• Cy5 / A647 (ex 628/40) em 692/40

Location: L.C.Miall 5.11, Faculty of Biological Sciences.