Jim Horne
- Email: bsjeah@leeds.ac.uk
- Supervisors: Professor Sheena Radford, Professor David Brockwell
Profile
PhD Student
Research interests
Using a variety of biophysical techniques, I study the mechanisms behind the folding and insertion of β-barrel outer membrane proteins (OMPs). My current focuses are:
- The interaction interfaces of OMPs with folding aids such as the periplasmic chaperones Skp and SurA, and the outer membrane protein folding catalyst, the β-barrel assembly machinery (BAM) complex.
- The mechanism of BAM / BamA-assisted OMP folding
- The super-molecular organisation of the BAM complex in vivo
To study these processes I exploit the power of cross-linking mass spectrometry, superresolution microscopy, and single-molecule and ensemble fluorescence spectroscopy.