Jim Horne


PhD Student

Research interests

Using a variety of biophysical techniques, I study the mechanisms behind the folding and insertion of β-barrel outer membrane proteins (OMPs). My current focuses are:

  • The interaction interfaces of OMPs with folding aids such as the periplasmic chaperones Skp and SurA, and the outer membrane protein folding catalyst, the β-barrel assembly machinery (BAM) complex.
  • The mechanism of BAM / BamA-assisted OMP folding
  • The super-molecular organisation of the BAM complex in vivo

To study these processes I exploit the power of cross-linking mass spectrometry, superresolution microscopy, and single-molecule and ensemble fluorescence spectroscopy.