How cells respond to stress: The role of chaperone post-translational modifications in health and disease

Supervisor(s)

Dr Anastasia Zhuravleva

Prof Richard Bayliss

 

Project description

The Hsp70 chaperones are key cellular molecular machines that protect organisms from the toxic consequences of environmental and pathological stresses. They perform a wide array of cellular functions: ensure that newly synthesised proteins are properly folded into their native structure, prevent protein misfolding and aggregation, assist translocation of organellar and secretory proteins, and control the activity of regulatory proteins. Growing evidence suggests that different Hsp70 functions can be precisely fine-tuned post-translationally to physiological requirements and specific cellular demands. However, much remains to be learned about molecular mechanisms of how exactly post-translational modifications control different Hsp70 functions.The main goal of this project is to characterise how stress and disease specific post-translational modifications affect Hsp70 structure, conformational flexibility, chaperone functional cycle and functions. To address this challenging biomedical problem, we are using a multidisciplinary approach that combines molecular biology (prokaryotic and eukaryotic protein production), the state-of-the-art structural techniques (nuclear magnetic resonance and X-ray crystallography), and computational methods (molecular dynamic simulations, coevolution analysis and modelling) .

 

 We are looking for an enthusiastic and ambitious PhD student with a strong interest in structural, computational and cellular biology. The successful candidate will be based at the Astbury Centre of Structural Molecular Biology and have access to our world-leading NMR facilities (including 950 MHz).

Entry requirements

Candidates should have, or be expecting, a 2.1 hons at Undergraduate level or above, in a relevant subject. If English is not your first language, you will need a recognised English Language qualification to be admitted onto any of the University's degree programmes: language requirements https://www.leeds.ac.uk/info/123100/admissions/143/entry_requirements

How to apply

Please apply online

Select “PhD in Biological Sciences” as the proposed course

Please include supervisor name and project title.  A research proposal is not required.  Please upload a CV and transcripts.

How to apply (email)

fbsgrad@leeds.ac.uk

How to apply (phone)

+44 (0)113 343 8186