Dr Eric Hewitt
- Position: Associate Professor
- Areas of expertise: Cell biology and immunology of amyloid disease; Alzheimer's; Parkinson's; dialysis-related amyloidosis; Lysosomes in health and disease
- Email: E.W.Hewitt@leeds.ac.uk
- Phone: +44(0)113 343 3030
- Location: 7.19C Miall
PhD from the University of Manchester in 1994, followed by postdoctoral work at the University of Dundee, University College London and the University of Cambridge. Appointed as a Lecturer at the University of Leeds in 2002.
Cell biology and immunology of amyloid disease
The formation of insoluble amyloid fibrils is associated with a spectrum of devastating human disorders, including Alzheimer’s, Parkinson’s, type 2 diabetes and dialysis related amyloidosis (DRA). In these disorders the formation of amyloid fibrils results in cellular dysfunction and tissue destruction. Understanding the mechanisms of amyloid toxicity is a priority if we are to develop new therapeutics for the amyloidoses.
Our goal is to determine how the structure and physical properties of amyloid fibrils and their assembly intermediates affects the function and viability of cells. This involves a highly collaborative and multidisciplinary approach in which information obtained structural biology techniques is integrated with cell biological analyses. Currently, we are studying the oligomeric assembly intermediates and fibrils formed by the amyloidogenic sequences, α-synuclein (Parkinson’s), amyloid-β (Alzheimer’s) and β2-microglobulin (DRA). We are examining the uptake of amyloid aggregates by cells, the pathological interactions between amyloid and cellular components, the induction of inflammatory responses, and the effects of amyloid on cellular physiology and cell viability. These experiments use an array of techniques, including plate-based assays for cell viability and metabolism, live cell confocal microscopy, flow cytometry, subcellular fractionation and proteomics. In addition, we are exploring a novel single molecule platform for the delivery of amyloid aggregates into the cytoplasm of cells with colleagues in the School Electrical and Electronic Engineering.
Lysosomes in health and disease
Lysosomes are membrane bound organelles whose functions include the degradation of cellular and extracellular material. We are studying the role of lysosomes in amyloid disease and our work suggests that this organelle is a key target for amyloid toxicity. In another project we are studying specialized lysosomes known as secretory lysosomes, which function as regulated secretory organelles in immune cells.<h4>Research projects</h4> <p>Any research projects I'm currently working on will be listed below. Our list of all <a href="https://biologicalsciences.leeds.ac.uk/dir/research-projects">research projects</a> allows you to view and search the full list of projects in the faculty.</p>
- BSc, Reading; PhD, Manchester
Postgraduate studentship areas:
- Cell biology of amyloid disease
- PGT Chair of Special Cases Committee - MCB programmes
- UG Chair of Special Cases Committee - MCB programmes
- Member of Undergraduate School Taught Student Education Committee
Research groups and institutes
- Cell and Organismal Biology
Current postgraduate researchers
<li><a href="//phd.leeds.ac.uk/project/436-an-integrated-approach-to-the-study-of-cellular-interactions-with-amyloid-in-neurodegenerative-disease">An integrated approach to the study of cellular interactions with amyloid in neurodegenerative disease</a></li>