Biomolecular Mass Spectrometry

Native MS

<h2 class="heading-underline">Native MS</h2>

<table cellpadding="0" cellspacing="0" style="width:500px;border: 0px solid white;margin-left:auto;margin-right:auto;" class="heading-underline"><tbody><tr><td style="border: 0px solid white"><img alt="Non-covalent (native) MS" src="/biologicalsciences/images/noncovalent.png" /></td><td style="text-align: left;vertical-align:text-top;border: 0px solid white"><strong>Useful for:</strong><ul><li>Determining complex stoichiometry</li><li>Assessing protein conformation</li><li>Characterising oligomerisation behaviour</li><li>Measuring CCS values using IMS-MS</li></ul></td></tr></tbody></table>

Mass spectrometry can be used to determine sub-unit stoichiometry and heterogeneity of protein complexes by using conditions that can preserve non-covalent interactions into the gas phase.

The success of a non-covalent MS measurement will depend upon the sample purity, concentration and the type of interaction predominating in the complex.

Electrostatic interaction tend to be enhanced in the absence of bulk solvent whereas hydrophobic interactions tend to be weakend.

Buffer exchange columns, spin dialysis filters and button dialysis options are available in the Facility and it is best to perform buffer exchange immediately prior to analysis where possible.

Sample concentration should be greater than 20 uM where possible and a minimal sample volume of 50 uL.

Sample submission

Samples can be submitted to the service at any time by bringing them to the Mass Spec Facility in lab 9.107 of the Astbury building.

You should download and complete a sample request form to bring along with your sample (please note that results of analysis cannot be made available until a grant code has been received).

Please note that by submitting samples to the MS Facility for analysis you agree to our conditions of service and publications policy. A copy of which can be found here.

Users external to the University can find additional information in the external users section.

Notes

The concentrations and volumes stated above are provided as a guide only. We will still accept and attempt MS analysis on samples of lower (or unknown) concentration and/or volume.