Super Resolution Microscopy
The resolution that can be achieved by traditional light microscopy is limited by the diffraction of light, which sets the shortest distance distinguishable between two points at ~half the wavelength of light (>200nm).
There are a diverse range of fundamental cellular processes that take place below the resolution limit of light and fluorescence microscopy. Super resolution microscopy uses a number of techniques to push beyond the 200nm barrier to enable the study of cellular structure and dynamic processes that couldn’t previously be imaged by standard imaging techniques.
Fluorescent super-resolution techniques enable highly specific, multi-colour imaging of both live and fixed cells.