Bio-imaging equipment

Lightsheet

The UltraMicroscope II uses 6 focused light sheets to excite cleared tissue samples, for gentle imaging through thick samples, on the µm to cm range.

Capabilities

  • 6 light sheets for homogeneous illumination, uni- and bi- directional illumination
  • Image in aqueous buffers and organic solvents (e.g. DBE, ECi, water)
  • Samples typically cleared with iDISCO technique
  • Zoom optic 0.63x – 6.3x
  • Z-stack and tile scanning
  • Sample travel range: 1cm x 1cm x 1cm
  • sCMOS camera

Microscope

  • UltraMicroscope II, LAVision Biotec
  • Olympus MVX10

Objectives

  • CDC 2x/0.5 with dipping cap (MV PLAPO 2XC) objective
  • 20x Dodt objective

Lasers

  • Ex. 470/40       Em. 525/50
  • Ex. 560/40       Em. 620/60
  • Ex. 630/30       Em. 680/30
  • Ex. 710/75       Em. 810/90

Location: Faculty of Biological Sciences, L.C.Miall 5.13.