Bio-imaging equipment

Confocal microscope for the imaging of both fixed and live cells. The inverted set-up enables samples to be imaged in a variety of holders including slides, chamber slides, imaging dishes or imaging plate formats.  

Capabilities

• Fully automated inverted microscope.
• 4 diode lasers (405, 488, 555, 639nm)
• Motorised stage for screening plates and slides, tile scanning and point visiting
• Z-sectioning for 3D imaging
• DIC and phase contrast objectives
• Temperature and CO2 regulation for live cell imaging
• FRAP and FRET imaging.

Microscope

• Axio Observer Z1 inverted stand
• Motorized stage: Marzhauser scan stage DC 120x100 mm
• Heated stage insert (Temperature)

Objectives

• 10x/0.3 Ph1 Plan-Neofluar, M27
• 20x/0.5 Ph2 Plan Neofluar, M27
• 40/1.3 Oil DIC Plan Apochromat, M27
• 63x/1.4 Oil DIC Plan Apochromat, M27
• 40x/1.2 water immersion Corrected, M27 (available on request)

Lasers

• 405nm laser, 5mW
• 488nm laser, 10mW
• 555nm laser, 10mW
• 639nm laser, 5mW

Spectral detector

• Two-channel collection (photomultipliers)
• Variable secondary dichroic (VSD) beamsplitter allows two simultaneous detections

Location

• NML Laboratory, Nexus Building.