Bio-imaging equipment

Confocal microscope for the imaging of both fixed and live cells. The inverted set-up enables samples to be imaged in a variety of holders including slides, chamber slides, imaging dishes, or imaging plate formats. Sensitive detectors with a high dynamic range enable imaging down to 120nm resolution.  

Capabilities

• Fully automated inverted microscope
• Improved resolution in x, y, and z, higher sensitivity and increased speed
• Spectral imaging and linear unmixing capabilities to allow the differentiation of similar fluorescence dyes or fluorescence signals from auto-fluorescence
• Motorised stage for screening plates and slides, tile scanning and point visiting
• Temperature and CO2 regulation for live cell imaging with fully integrated incubator box
• DIC imaging
• Polarised imaging
• FRAP imaging including FRAP analysis module
• Reflective imaging capabilities
• Photon counting modes
• Super-resolution mode for 120nm resolution

Microscope

• Olympus IX83 microscope stand
• CellVivo incubator and vivo stagetop incubator (temperature, humidity and Co2 control).
• Truefocus - autofocus devise for focus tracaking

Lasers

Laser lines: 405nm, 445nm, 488nm, 514nm, 561nm, 594nm, 640nm, 685nm, 730nm, 785nm

Objectives

• 4x/0.16 air,
• 10x/0.4 air,

• 20x/0.8 air,
• 40x/1.4 oil,
• 60x/1.5 oil
• 25x/0.85 Silicon pad (available on request)
• 40x/1.25 Silicon oil (available on request)
• 60x/1.3 Silicon oil (available on request)

Detectors and Emission filters

• Galvo and RASTA scanners of fast scanning options
• Tuneable emission filters (400-900nm)
• 6 SilvR Detectors with high dynamic range
• Photon counting mode

Location: Bioimaging Facility, Roger Stevens 5.52M