Bio-imaging equipment

Confocal microscope for the imaging of both fixed and live cells. The inverted set-up enables samples to be imaged in a variety of holders including slides, chamber slides, imaging dishes or imaging plate formats.  

Capabilities

• Fully automated inverted microscope.
• Improved resolution in x, y and z, higher sensitivity and increased speed
• Spectral imaging and linear un-mixing capabilities to allow the differentiation of similar fluorescence dyes or fluorescence signals from auto-fluorescence.
• Sensitive GaAsp detectors allow you to use less laser power, performing gentle imaging of your samples
• Motorised stage for screening plates and slides, tile scanning and point visiting
• Piezo stage for faster and more accurate Z-stacks and 3D imaging
• Temperature and CO2 regulation for live cell imaging with heated stage and fully integrated incubator box
• FRAP and FRET imaging including FRAP analysis module
• FCS (Fluorescence correlative spectroscopy) capabilities
• 1.7x higher resolution in all three dimensions with Fast Airyscan, compared to conventional confocal microscopy

Microscope

• Axio Observer.Z1
• Nano Focusing Piezo Stage
• ZL multi S1 Incubator box (Temperature and CO2)
• Heated stage insert (Temperature and CO2)

Lasers

• Diode 405 nm
• Argon 458, 488, 514 nm
• DPSS 561 nm
• HeNe 633 nm

Objectives

• EC Plan-Neofluor 10x/0.30
• Plan-Apochromat 20x/0.8
• C-Apochromat 40x/1.2 W Korr FCS (available on request)
• Plan-Apochromat 40x/1.4 Oil DIC
• Plan-Apochromat 63x/1.4 Oil DIC

PMTs and Emission Filters

• 2 PMTs plus additional GaAsp detector with fully 'tunable' emmision collection

• Fast Airyscan module for 1.7x increased resolution with the following emission filters:
  • BP420-480 + BP495-550
  • BP420-480 + BP495-620
  • BP420-480 + LP605
  • BP465-505 + LP525
  • BP495-550 + LP570
  • BP570-620 + LP645

Location

Faculty of Biological Sciences, L.C.Miall 5.08